Journal: Skin Research and Technology

Article Title: Micropuncture closure following high density microarray patch application in healthy subjects

doi: 10.1111/srt.13131

Figure Lengend Snippet: To assess the possible effects of histamine on the degree of erythema within the high‐density microarray patch (HD‐MAP) application area, TiVi data before and after histamine challenge were compared using repeated‐measures one‐way ANOVA, mixed effect analysis, α = 0.05. Asterisks mark significant changes

Article Snippet: TEWL and TiVi data before and after histamine challenge were compared using repeated‐measures one‐way analysis of variance (ANOVA), mixed effect analysis, α = 0.05 (GraphPad Prism version 9.1.2 for Windows, GraphPad Software, San Diego, California USA, www.graphpad.com ).

Techniques: Microarray

Journal: Oncotarget

Article Title: The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models

doi: 10.18632/oncotarget.24539

Figure Lengend Snippet: (A) Healthy human subjects were studied in-laboratory and subjected to 3 days of a simulated day shift schedule ( B , control condition) or a simulated night shift condition ( C , experimental condition). This was followed by a 24-hour constant routine protocol during which blood was drawn at 3-hour intervals (ZT2, ZT5, etc.) Blood samples collected at ZT2 and ZT14 (8 AM and 8 PM, respectively, in the day shift condition, or 8 PM and 8 AM in the night shift condition) were immediately treated with 10 μM cisplatin. Blood samples were incubated and fractions were collected between 2 and 24 hours later to isolate PBMCs. Genomic DNA was purified and probed for cisplatin-DNA adduct levels with an α-Pt-(GpG) antibody using a slot-blot assay. (D) mRNA was isolated from the blood samples and gene expression for XPA, the rate-limiting factor in NER, was analyzed using the NanoString multiplex assay. (E) DNA-protein interaction between the Bmal1 and XPA is shown in the first 3,000 base pair promoter region of human melanoma SKMEL-27 cells using a ChIP assay. PER2 is a circadian clock positive control. Input and IgG are experimental positive and negative controls, respectively. (F) Quantitation of Bmal1 binding to promoter regions of PER2 and XPA from ChIP assay, indicating regions of significance after IgG binding subtraction. Statistical analysis was done using two-way ANOVA with n=3 subjects per group (B-C) and cosinor analysis with n=7 subjects per group (D), and t test with n=3 replicates for E-F. * =p<0.05 for circadian rhythmicity or ChIP binding. Error bars = S.E.M.

Article Snippet: Oscillations of genes were analyzed using mixed-effects cosinor analysis (SAS software) [ ].

Techniques: Control, Incubation, Purification, Slot Blot Assay, Isolation, Gene Expression, Multiplex Assay, Positive Control, Quantitation Assay, Binding Assay

Journal: Scientific Reports

Article Title: Physiological and economic benefits of abandoning invasive surgical procedures and enhancing animal welfare in swine production

doi: 10.1038/s41598-019-52677-6

Figure Lengend Snippet: Injuries, weakness and mortality of piglets/pigs as husbandry invasive surgical procedures were gradually avoided and meaningful environmental enrichment was provided. ( a ) Mixed effects Poisson Regression Coefficient analysis of injuries occurrence in Groups 2-4 (G2-4) as compared to the control group (Group 1; G1); data is presented as coefficient (sign) and 95% confidence interval (bars). ( b ) Odds Ratio to be weak or dead in Groups 2-4 (G2-4) as compared to the control group (Group 1); data was analyzed by a chi-square goodness of fit test and the Binominal test, and is presented as Odds Ratio (sign) and 95% confidence interval (bars). In both panels, Group 1 is represented by the horizontal dotted line; values significantly differed ( P < 0.05) from Group 1 if the bar does not cross the dotted line.

Article Snippet: Figure 2 Injuries, weakness and mortality of piglets/pigs as husbandry invasive surgical procedures were gradually avoided and meaningful environmental enrichment was provided. ( a ) Mixed effects Poisson Regression Coefficient analysis of injuries occurrence in Groups 2-4 (G2-4) as compared to the control group (Group 1; G1); data is presented as coefficient (sign) and 95% confidence interval (bars). ( b ) Odds Ratio to be weak or dead in Groups 2-4 (G2-4) as compared to the control group (Group 1); data was analyzed by a chi-square goodness of fit test and the Binominal test, and is presented as Odds Ratio (sign) and 95% confidence interval (bars).

Techniques: Control

Journal: Scientific Reports

Article Title: Physiological and economic benefits of abandoning invasive surgical procedures and enhancing animal welfare in swine production

doi: 10.1038/s41598-019-52677-6

Figure Lengend Snippet: Hair and saliva cortisol concentrations in piglets/pigs, as husbandry invasive surgical procedures were gradually avoided and meaningful environmental enrichment was provided. Individual hair cortisol concentrations at weaning ( a ) and slaughter ( b ) were analyzed by Multi Variate Linear regression test (controlled for variables: mother cortisol, weaning weight and suckling period). Saliva was collected for cortisol measurements at the pen level, every two weeks from weaning to slaughter, and the results are presented in ( c ) and ( d ). ( c ) Samples were clustered together per group, without taking the sample date into consideration; a mixed effect linear regression model was used for statistical analysis (Random effect: pen. Predictor: treatment group. Adjusted for: tail biting, leg injuries, skin lesions, weakness and survival). ( d ) Analysis of saliva cortisol in Group 2-4, as compared to Group 1; for the statistical analysis, time varying group effects was applied, using log-level regressions on group identifiers and individual treatments, standard errors were clustered at the pen level. In all panels, data is presented as coefficient (sign) and 95% confidence interval (bars); Group 1 is represented by the horizontal dotted line; values significantly differed ( P < 0.05) from Group 1 if the bar do not cross the dotted line.

Article Snippet: Figure 2 Injuries, weakness and mortality of piglets/pigs as husbandry invasive surgical procedures were gradually avoided and meaningful environmental enrichment was provided. ( a ) Mixed effects Poisson Regression Coefficient analysis of injuries occurrence in Groups 2-4 (G2-4) as compared to the control group (Group 1; G1); data is presented as coefficient (sign) and 95% confidence interval (bars). ( b ) Odds Ratio to be weak or dead in Groups 2-4 (G2-4) as compared to the control group (Group 1); data was analyzed by a chi-square goodness of fit test and the Binominal test, and is presented as Odds Ratio (sign) and 95% confidence interval (bars).

Techniques: